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Establishing an Artificial Pathway for De Novo Biosynthesis of Vanillyl Alcohol in Escherichia coli.

ACS synthetic biology (2017-06-07)
Zhenya Chen, Xiaolin Shen, Jian Wang, Jia Wang, Ruihua Zhang, Justin Forrest Rey, Qipeng Yuan, Yajun Yan
ABSTRAKT

Vanillyl alcohol is a phenolic alcohol and is used as a flavoring agent in foods and beverages. In this paper, we propose a novel artificial pathway for microbial production of vanillyl alcohol from simple carbon sources. The pathway extends from 4-hydroxybenzoic acid (4-HBA), and needs only three heterologous enzymes, p-hydroxybenzoate hydroxylase (PobA), carboxylic acid reductase (CAR) and caffeate O-methyltransferase (COMT). First, we examined the promiscuous activity of COMT toward 3,4-dihydroxybenzyl alcohol and found a k

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Sigma-Aldrich
3-Hydroxy-4-methoxybenzyl alcohol, 98%