Evaluation of hydrophilic interaction liquid chromatography-tandem mass spectrometry and extraction with molecularly imprinted polymers for determination of aminoglycosides in milk and milk-based functional foods.

An analytical method for the determination of eleven aminoglycosides in different types of milk and milk-based functional products has been optimized and validated. A hydrophilic interaction chromatography (HILIC) column was proposed for the separation of analytes by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A commercially molecularly imprinted polymer has been used for the solid phase extraction of the analytes, in order to achieve high selectivity in the sample treatment. The proposed method was characterized for different types of milk (whole cow milk, skimmed cow milk, whole goat milk) and functional dairy products, such as follow-on milk, Omega 3-enriched milk and isoflavones-enriched milk. Matrix effect was studied in the different matrices, being lower than │15│% in all cases, showing that the proposed procedure provided very clean extracts. Limits of quantification in the range 4.2-49µgkg