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In vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes.

Journal of bacteriology (1993-01-01)
H Wada, H Schmidt, E Heinz, N Murata
ABSTRAKT

Thylakoid membranes isolated from the cyanobacterium Synechocystis sp. strain PCC6803 were capable of desaturating the acyl groups in monogalactosyl diacylglycerol. This desaturation reaction required the reduced form of ferredoxin.

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Sigma-Aldrich
β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥93% (HPLC)
Sigma-Aldrich
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach), lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase
Sigma-Aldrich
Transcreener® ADP2 FI Assay