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[Pathogenic properties of fungi Fusarium genus isolated from patients with atopic dermatitis].

Zhurnal mikrobiologii, epidemiologii, i immunobiologii (2013-09-05)
S A Lisovskaia, N I Glushko, E V Khaldeeva
ABSTRAKT

Study of adhesive activity and growth speed of fungi Fusarium genus isolated from the surface of cutaneous covering in patients with atopic dermatitis (AD). Clinical and museum F. oxysporum, F. solani strains were used in the study. Seeding was carried out into agarized Czapek and Sabouraud medium. The cultures were studied for9 days at 30 +/- 2 degrees C, adhesive properties of fungi were determined by using model based on nitrocellulose film (S.A. Lisovskaya et al., 2006). The studies carried out showed statistically significant differences in adhesive properties and intergrowth intensity of various Fusarium spp. Adhesion level of F. solani microconidia was almost 2.5 times higher compared with F. oxysporum. Formation of the first germ tube by F. solani microconidia was noted during the first 10 hours whereas by F. oxysporum--only at day 2. The data obtained confirm species differences of Fusarium spp. pathogenic properties that emphasize the importance of species identification of fungi.

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Collodion solution, 4-8% in ethanol/diethyl ether
Sigma-Aldrich
Collodion solution, for microscopy, 2% in amyl acetate