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Merck

Use of conventional bioanalytical devices to automate DBS extractions in liquid-handling dispensing tips.

Bioanalysis (2011-10-21)
Casey Jl Johnson, Chad D Christianson, Chrystal N Sheaff, Derek F Laine, Jennifer Sd Zimmer, Shane R Needham
ABSTRAKT

Conventional liquid-handling devices were employed, along with an improved punching device, to semi-automate dried blood spot (DBS) extraction of alprazolam, α-hydroxyalprazolam and midazolam from human whole blood. Liquid-handling devices were used to add internal standard to the DBS cards and to extract the analytes from the DBS, in order to be analyzed by HPLC-MS/MS. The technique was shown to be accurate (±12.0%) and precise (10.3%) across the dynamic range of the assay. The semi-automated extraction reduced sample preparation time by more than 50% when compared with more conventional DBS manual extraction methods.

MATERIAŁY
Numer produktu
Marka
Opis produktu

Supelco
alpha-Hydroxyalprazolam solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Sigma-Aldrich
α-Hydroxyalprazolam, powder, ≥98% (TLC)