- The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis.
The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis.
The Journal of eukaryotic microbiology (2018-08-05)
Christiane B de Araujo, Simone G Calderano, Maria Carolina Elias
PMID30076751
ABSTRAKT
Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although T. cruzi exhibits a mean fork speed (2.05 ± 0.10 kb/min) very similar to other trypanosomatids, we found that the majority of DNA molecules replicated more slowly, with a frequency distribution approximately 1 kb/min. This frequency distribution analysis provides more information about the replication profile of this organism.
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