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Merck

Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures.

Methods in molecular biology (Clifton, N.J.) (2021-05-08)
Hirotaka Watanabe, Rei Murakami, Hideyuki Okano
ABSTRAKT

Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.

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Sigma-Aldrich
β-Secretase Inhibitor IV, β-Secretase Inhibitor IV, CAS 797035-11-1, is a cell-permeable inhibitor that binds to BACE-1 active site and blocks its proteolytic activity (IC₅₀ = 15 nM for human BACE-1).
Sigma-Aldrich
DAPT, ≥98% (HPLC), solid