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Properties of a milk clotting protease isolated from fruits of Bromelia balansae Mez.

Biological chemistry (2001-08-24)
M F Pardo, L M López, N O Caffini, C L Natalucci
ABSTRAKT

Unripe fruit extracts of Bromelia balansae Mez (Bromeliaceae), whose principal endopeptidase is balansain I (isolated for anion exchange chromatography: pI = 5.45, molecular weight = 23192), exhibit a pH profile with a maximum activity around pH 9.0 and are inhibited only by cysteine peptidases inhibitors. The alanine and glutamine derivatives of N-alpha-carbobenzoxy-L-amino acid p-nitrophenyl esters were strongly preferred by the enzyme. Enzymatic hydrolysis of milk and soy proteins yield characteristic patterns at pH 9.0. The N-terminal sequence showed a very high homology (85-90%) with other known Bromeliaceae endopeptidases.

MATERIAŁY
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Opis produktu

Sigma-Aldrich
Z-L-Lys-ONp hydrochloride