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Merck

High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples.

STAR protocols (2020-10-29)
Emily M Thrash, Katja Kleinsteuber, Emma S Hathaway, Matthew Nazzaro, Eric Haas, F Stephen Hodi, Mariano Severgnini
ABSTRAKT

As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important. Here, we present a streamlined MC workflow for high-throughput staining that generates reproducible data for up to 80 samples in a single experiment by combining reference sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive, this workflow decreases experimental variables and thus reduces technical error and mitigates batch effects.

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Sigma-Aldrich
Bovine Serum Albumin solution, 30%±2% in 0.85% sodium chloride, aseptically filled