T4532

Tryptose Broth

suitable for insect cell culture

• Kod UNSPSC: 41106507
• NACRES: NA.75

Właściwości

• pochodzenie biologiczne: Porcine pancreas
• Postać: liquid
• metody: cell culture | insect: suitable
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.

Komponenty

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Inne uwagi

This Tryptose broth is closely related to Tryptose phosphate broth, but does not contain the phosphate component and it contains a lower concentration of dextrose.

Uwaga dotycząca przygotowania

This formulation contains Tryptose (20 g/L); Dextrose (1 g/L); and NaCl (5g/L) adjusted to a final pH of 7.2.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable