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Key Documents

C-23130

Sigma-Aldrich

Fibroblast Growth Medium 3

Kit including Basal Medium and SupplementPack, 500 ml

Synonim(y):

Fibroblast Culture Medium, Fibroblast Medium

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.71

Postać

liquid

opakowanie

pkg of 500 ml

metody

cell culture | mammalian: suitable

Warunki transportu

wet ice

Opis ogólny

Fibroblast Growth Medium 3 Kit

Zastosowanie

PromoCell Fibroblast Growth Medium 3 has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF). The medium is optimized for primary human cells but can also be used for porcine cardiac fibroblasts.Recommended for:Human Cardiac Fibroblasts (HCF)

Komponenty

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Jakość

All lots of PromoCell Fibroblast Growth Medium 3 are subjected to comprehensive quality control tests using primary Human Cardiac Fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved inhouse lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Uwaga dotycząca przygotowania

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.1 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Przechowywanie i stabilność

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementMix and SupplementPack are delivered thawed and can be frozen after arrival without losing any activity.

Rutyna subkultury

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Kod klasy składowania

10 - Combustible liquids


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Produkty

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

Pierwotne ludzkie fibroblasty i pożywka wzrostowa do hodowli in vitro młodych i dorosłych fibroblastów. Protokoły obsługi, pasażowania komórek, pożywki i stosowania produktu.

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