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W1754

Sigma-Aldrich

Water

PCR Reagent, suitable for PCR

Synonim(y):

H2O

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About This Item

Wzór liniowy:
H2O
Numer CAS:
7732-18-5
Masa cząsteczkowa:
18.02
Beilstein:
2050024
Numer WE:
231-791-2
Numer MDL:
MFCD00011332
Kod UNSPSC:
12191602
Identyfikator substancji w PubChem:
24900779
NACRES:
NA.25

product name

Water, PCR Reagent

klasa czystości

PCR Reagent

Poziom jakości

200

gęstość pary

<1 (vs air)

ciśnienie pary

3 mmHg

sterylność

sterile-filtered

Postać

liquid

opakowanie

vial of 1.5 mL

metody

PCR: suitable

współczynnik refrakcji

n20/D 1.34 (lit.)

pH

5-7

tw

100 °C (lit.)

mp

0 °C (lit.)

gęstość

1.000 g/mL at 3.98 °C (lit.)

obecność zanieczyszczeń

DNase, none detected
RNase, none detected

ciąg SMILES

O

InChI

1S/H2O/h1H2

Klucz InChI

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Opis ogólny

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Zastosowanie

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Przydatność

Suitable for polymerase chain reaction (PCR)

Inne uwagi

Easily compare specifications for Water products with the Water specification table.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
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Protokoły

sybr green jumpstart taq readymix

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Hot Start dNTP protocol to reduce non-specific amplification

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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