17-10078
EZ-Magna ChIP® HT96 Chromatin Immunoprecipitation Kit
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About This Item
Polecane produkty
metody
immunoprecipitation (IP): suitable
Poziom jakości
Opis ogólny
The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative control antibodies and validated primer set plus a detailed protocol.
Zastosowanie
The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.
Cechy i korzyści
- Complete set of materials for up to 96 ChIP reactions
- Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
- Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
- Includes negative and positive control antibodies and control primer set to simplify validation of experimental procedure
- Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systemsProtocols for using cells or tissues
- Direct analysis of DNA without additional clean-up steps
- Compatible with ChIPAb+ validated antibody and primer sets
Opakowanie
Kit capacity: 96 chromatin immunoprecipitation assays
Komponenty
Magna ChIP protein A/G magnetic beads;HT96 Nuclei Isolation Buffer ;HT96 ChIP Buffer (Sonication/ChIP/Wash);Low Stringency IP Wash Buffer ;HT96 ChIP Elution Buffer ;Proteinase K Solution;Protease Inhibitor Cocktail III;10X Glycine ;10X PBS ;96 Well ChIP Plate ;96 Well Thermal Plate;Plate Seal;Strip Caps;Anti-Trimethyl-Histone H3 (Lys4) -positive control antibody;Normal Rabbit IgG -negative control antibody;GAPDH Primers - PCR positive control
Informacje prawne
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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