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Merck

L2897

Sigma-Aldrich

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Synonim(y):

Lennox broth, Luria Bertani Agar

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About This Item

Kod UNSPSC:
41106200
NACRES:
NA.85

klasa czystości

for molecular biology

Poziom jakości

sterylność

non-sterile

Formularz

powder

skład

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

metody

microbiological culture: suitable

Zastosowanie

food and beverages
microbiology

temp. przechowywania

room temp

przydatność

nonselective for Escherichia coli
nonselective for coliforms

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Opis ogólny

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Zastosowanie

LB Broth with agar (Lennox) has been used for:

  • the transformation of competent E. coli cells
  • to cultivate Ecc-15 bacteria
  • embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
  • transmission electron microscopy (TEM) and immunohistochemistry (IHC)
It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Cechy i korzyści

Lennox LB powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Easy scale-up using larger package sizes
  • Standard formulation

Uwaga dotycząca przygotowania

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Informacje prawne

Zeocin is a registered trademark of Cayla Sarl
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Farbod Alimohammadi et al.
Langmuir : the ACS journal of surfaces and colloids, 34(24), 7192-7200 (2018-05-22)
Two-dimensional (2D) nanomaterials have attracted considerable attention in biomedical and environmental applications due to their antimicrobial activity. In the interest of investigating the primary antimicrobial mode-of-action of 2D nanomaterials, we studied the antimicrobial properties of MnO2 and MoS2, toward Gram-positive
Patricia E Carreira et al.
Mobile DNA, 7, 21-21 (2016-11-16)
LINE-1 (L1) retrotransposons are a notable endogenous source of mutagenesis in mammals. Notably, cancer cells can support unusual L1 retrotransposition and L1-associated sequence rearrangement mechanisms following DNA damage. Recent reports suggest that L1 is mobile in epithelial tumours and neural
Martin Wegner et al.
Bio-protocol, 10(1), e3472-e3472 (2020-01-05)
Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA
Shun Gen Huang et al.
BMC immunology, 14, 27-27 (2013-06-22)
Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible
Luis Ontiveros-Padilla et al.
Microorganisms, 9(4) (2021-05-01)
Salmonella enterica serovar Typhi (S. Typhi) porins, OmpC and OmpF, are potent inducers of the immune response against S. Typhi in mice and humans. Vaccination with porins induces the protection against 500 LD50 of S. Typhi, life-lasting bactericidal antibodies and

Protokoły

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

General protocols for growth of competent cells in microbial medium.

Hodowla bakterii E. coli w skali od małych kultur do fermentacji. Uwzględniono protokoły inicjowania kultury starterowej, komórek zawiesinowych, monokultury i posiewu bakteriofaga M13.

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