MIRRM03
MystiCq® microRNA® SYBR® Green qPCR ReadyMix™
Formulation for miRNA RT-qPCR on iQ Bio-Rad platforms
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
compatibility
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
storage temp.
−20°C
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General description
The MystiCq® microRNA SYBR® Green qPCR ReadyMix™ is one of the integral components of the MystiCq microRNA qPCR Assay System. This master mix is a specially formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.
Application
MystiCq® microRNA SYBR® Green qPCR ReadyMix™ may be used in reverse transcription-quantitative polymerase chain reaction (RT-PCR) to measure miRNA expression levels in different cell lines.
Features and Benefits
- Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
- 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
- Passive reference dye (if required) included at the optimal concentration
- This master mix can readily amplify fragments between 400 and 500 bp; however, for best results, amplicon size should be limited to 80 - 200 bp
Packaging
Default reaction volume is 50 μL
100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL
100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
MystiCq® microRNA SYBR® Green qPCR ReadyMix™, iQ™, is compatible with the following platforms: Biorad iCycler iQ, BioRad iQ5, BioRad MyiQ™.
Legal Information
MyiQ is a trademark of Bio-Rad Laboratories, Inc.
MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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