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Z722553

ThermalSeal RT2RR™ film

non-sterile, sealing films for real-time qPCR

Synonym(s):

ThermalSeal RT™ film, real-time qPCR plate seal

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About This Item

UNSPSC Code:
41122410
NACRES:
NB.22

material

polyester film

sterility

non-sterile

packaging

pack of 100 ea

manufacturer/tradename

Excel Scientific TS-RT2RR-100

technique(s)

PCR: suitable
qPCR: suitable

L

130.6 mm

W × L

7.78 cm × 13.08 cm

W

77.8 mm

color

clear

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General description

A combination of optically transparent polyester film with a strong, ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications. In place of the customary paper backing, ThermalSeal RT has a plastic liner which is easily removed before use and contributes smoothness and extreme optical clarity to the adhesive. Dimensions are 3.125 x 5.625 in. to seal PCR plates. Length between the perforated ends is 4.8 in.
  • Ultra-high optical clarity
  • Heat resistant, recommended for temperatures from -40°C to +120°C
  • Certified DNase, RNase, and nucleic acid free
ThermalSeal RT2RR™ 50 μm polyester sealing films for real-time PCR are sized to fit within the edges of raised-rim 96 well plates. The same consistent ultra-high optical clarity as Excel′s ThermalSeal RT™ sealing films makes possible more reproducible, reliable, and consistent DNA amplification measurements. An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. Two end tabs assist in positioning the sealing film. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
  • Ultra-high optical clarity
  • Fit within plate rim to prevent evaporation due to sealing film lifting
  • Heat resistant, recommended for temperatures from
  • -40 °C to +120 °C
  • Certified DNase, RNase, and Nucleic Acid free

Legal Information

ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RT is a trademark of Excel Scientific, Inc.
ThermalSeal RT2RR is a trademark of Excel Scientific, Inc.

Certificates of Analysis (COA)

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Protocols

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Multiplex qPCR Protocol

Although quantitative PCR uses the same basic concept as traditional PCR, the reactions differ in that the amplicons are generally smaller and are detected indirectly using an additional dye or labeled probe or primer.

Standard Reverse Transcription Protocol (Two-step)

reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.

qPCR Using a Single Detection Probe Protocol

A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.

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