Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

62197

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat

~1 mg/mL

Synonym(s):

Atto 488 Anti-Mouse IgG, Goat Anti-Mouse IgG

Sign Into View Organizational & Contract Pricing

Select a Size

1 ML
424,00 €

424,00 €

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(1)

Select a Size

Change View
1 ML
424,00 €

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

424,00 €

Please contact Customer Service for Availability
Request a Bulk Order

biological source

goat

Quality Level

100

conjugate

Atto 488 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

mouse

concentration

~1 mg/mL

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A3438A1047A9316
Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat ~1 mg/mL

62197

Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina affinity isolated antibody, buffered aqueous glycerol solution

A3438

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina affinity isolated antibody, buffered aqueous solution

A1047

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina

Fosfatasi alcalina anti-IgG murina (molecola intera)− affinity isolated antibody, buffered aqueous glycerol solution

A9316

Fosfatasi alcalina anti-IgG murina (molecola intera)−

biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

immunocytochemistry: 1:500, immunofluorescence: 1:400

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:7,000-1:21,000, western blot: suitable

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

Atto 488 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.

Immunogen

purified mouse IgG.

Application

Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:
  • in immunofluorescence confocal microscopic assay for p65 localization
  • in immunohistochemical analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).

Physical form

Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCG0706
    BCCF1219
    BCCD1950
    BCCC9311
    BCCB9457

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    IgG subclasses and allotypes: from structure to effector functions
    Vidarsson G, et al.
    Frontiers in Immunology, 5(1-2), 520-520 (2014)
    Arnold Garbiec et al.
    Arthropod structure & development, 41(2), 165-176 (2012-02-04)
    Mechanisms that underlie differentiation and diversification of the ovarian follicular epithelium in insects have been best characterized in a fruit fly, Drosophila melanogaster. Recent comparative analyses have shown that dipterans evolved a common, specific system of early patterning of their
    Sumeda Nandadasa et al.
    PloS one, 7(6), e38756-e38756 (2012-06-22)
    Alpha (α)-E-catenin is a component of the cadherin complex, and has long been thought to provide a link between cell surface cadherins and the actin skeleton. More recently, it has also been implicated in mechano-sensing, and in the control of
    Emily J Reedich et al.
    Journal of neuroscience research, 100(10), 1951-1966 (2022-07-16)
    Spastic cerebral palsy (CP) is a movement disorder marked by hypertonia and hyperreflexia; the most prevalent comorbidity is pain. Since spinal nociceptive afferents contribute to both the sensation of painful stimuli as well as reflex circuits involved in movement, we
    Carolyn L Smith et al.
    The Journal of general physiology, 149(4), 483-510 (2017-03-24)
    Four-domain voltage-gated Ca2+ (Cav) channels play fundamental roles in the nervous system, but little is known about when or how their unique properties and cellular roles evolved. Of the three types of metazoan Cav channels, Cav1 (L-type), Cav2 (P/Q-, N-
    View All Related Papers

    Articles

    Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

    Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service