D9564

DAPI, dilactate

≥98% purity (HPLC), powder

• Synonym(s): 4′,6-Diamidino-2-phenyindole, dilactate
• Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2C₃H₆O₃
• Molecular Weight: 457.48
• UNSPSC Code: 12171500
• PubChem Substance ID: 329798905
• NACRES: NA.47

Properties

• product name: DAPI, dilactate, ≥98% (HPLC)
• Quality Level: 300
• Assay: ≥98% (HPLC)
• form: powder
• color: yellow
• solubility: H₂O: 20 mg/mL
• ε (extinction coefficient): 28.00-32.50 at 345-351 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)
• InChI key: LCAUPANIXBMNIA-UHFFFAOYSA-N

Description

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves