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48300

Millipore

Bile esculin agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Esculin Bile Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

Agency

according to ISO 10273:2017

Quality Level

200

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
esculin, 1 g/L
ferric citrate, 0.5 g/L
meat extract, 3 g/L
meat peptone, 5 g/L
ox-bile, 40 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable

final pH

6.6±0.2 (25 °C)

application(s)

environmental
food and beverages
water monitoring
microbiology

suitability

Streptococcus spp.
selective and differential for Enterococcus spp.

Application

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.
For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970).

Preparation Note

Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD9566
BCCB8736
BCBW5478
BCBV7559
BCBT5798

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Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation. Microbiology notes. Pratiksha Pokhrel September 1, 2015 Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation2015-10-16T17:40:41+00:00 Bacteriology, Biochemical Test, Diagnosis, Laboratory.
Science (2015)
Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.
Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn
Journal of Clinical Microbiology, 1 (5), 440-443 (1975)
R R Facklam et al.
Applied microbiology, 20(2), 245-250 (1970-08-01)
Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for
The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).
A SWAN
Journal of clinical pathology, 7(2), 160-163 (1954-05-01)

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