17185

m-Enterococcus Agar, modified

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Membrane filter Enterococcus Agar, modified
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; cycloheximide, 0.05 g/L ; esculin, 1 g/L ; nalidixic acid, 0.25 g/L ; pancreatic digest of gelatin, 10 g/L ; sodium azide, 0.15 g/L ; sodium chloride, 15 g/L ; yeast extract, 30 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.1±0.2 (25 °C)
• application(s): agriculture; environmental; food and beverages; water monitoring; microbiology
• suitability: slightly selective and differential for Enterococcus spp.

Description

Application

For the recovery of Enterococci in water samples using membrane filter technique along with Esculin Iron Agar for the identification.

Preparation Note

Suspend 71.4 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45°C and aseptically add 15 ml of sterile 1% TTC Solution Cat. No. No. 17779). Mix well and pour into sterile petri plates. Warning: Cycloheximide is very toxic. Avoid skin contact or aerosol formation and inhalation.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves