PMEF-CFL-P1

EmbryoMax^® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Not Treated, Passage 1

• Synonym(s): CF-1 MEF Feeder Cells, CF-1 MEFs, CF-1 PMEFs, CF1 Mouse Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
• UNSPSC Code: 41106509
• eCl@ss: 32011203

Properties

• biological source: mouse
• Quality Level: 100
• manufacturer/tradename: Specialty Media; EmbryoMax^®
• technique(s): cell culture | stem cell: suitable
• input: sample type induced pluripotent stem cell(s); sample type primary embryotic fibroblasts (PMEFs); sample type: mouse embryonic stem cell(s)
• shipped in: liquid nitrogen

Description

General description

PMEF-CFL-P1 are low passage Primary Mouse Embryo Fibroblasts (PMEFs) isolated from CF1 mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

Cell Line Description

Primary Mouse Embryo Fibroblasts

Application

PMEF, Strain CF1, Not Treated, Passage 1

Research Category
Stem Cell Research

Packaging

1x10⁶ cells

Physical form

Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.

Storage and Stability

After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable