ESG1106
ESGRO® Recombinant Mouse LIF Protein
ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml
Synonym(s):
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160801
NACRES:
NA.75
Recommended Products
Quality Level
Assay
>95% (active component mLIF, SDS-PAGE)
form
liquid
manufacturer/tradename
Chemicon®
concentration
1000000 units/mL
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
mouse ... LIF(16878)
General description
Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
Application
Applications for ESGRO Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3).
At the recommended concentration 107 units of ESGRO Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3).
At the recommended concentration 107 units of ESGRO Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.
ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml
Physical form
ESGRO Supplement is supplied in liquid form as 106 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Format: Purified
Preparation Note
ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
Storage and Stability
ESGRO Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
Analysis Note
Specific Activity: ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO Supplement in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 106 units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media.
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Ashley L Fritz et al.
Biotechnology and bioengineering, 112(1), 209-219 (2014-07-30)
The induction of pluripotency from adult cells has enormous potential in regenerative medicine. While initial efforts to study mechanisms and improve efficiency of induced pluripotent stem cell (iPSC) reprogramming focused on the direct roles of transcriptional regulators, increasing evidence indicates
Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification.
Ito S. et al.
Nature null
A conditional knockout resource for the genome-wide study of mouse gene function
Skarnes W. C. et al.
Nature null
L Ferraris et al.
Nucleic acids research, 39(6), e33-e33 (2010-12-21)
There are a variety of in vivo and in vitro methods to determine the genome-wide specificity of a particular trans-acting factor. However there is an inherent limitation to these candidate approaches. Most biological studies focus on the regulation of particular
Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells.
Andrade, Pedro Z, et al.
Molecular Biosystems, 6, 1207-1215 (2010)
Articles
Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors.
Protocols
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
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