SAB4200775
Anti-Glycoprotein (MDR) antibody, Mouse monoclonal
clone F4, purified from hybridoma cell culture
Synonym(s):
ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
F4, monoclonal
form
buffered aqueous solution
species reactivity
hamster, human
concentration
~1 mg/mL
technique(s)
flow cytometry: suitable
immunoblotting: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ABCB1(5243)
General description
Anti-P-Glycoprotein (MDR) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma F4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Multidrug resistance protein 1 (MDR1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporters superfamily. It is ubiquitously expressed in kidneys, intestines, placenta, liver, adrenal glands blood-brain barrier (BBB), placenta and testis.
Specificity
Anti-P-Glycoprotein (MDR) antibody, Mouse monoclonal recognizes an epitope located at the N-terminal region of P-glycoprotein (Pgp), at the third extracellular loop of the molecule, also known as ABCB1 protein. The antibody specifically detects MDR1 P-glycoprotein, but does not recognize MDR3. Anti-P-Glycoprotein (MDR) cross reacts with MDR1 P-glycoprotein from human and hamster origin.
Immunogen
a mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
Application
Anti-Glycoprotein (MDR) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemistry
- cellular enzyme-linked immunosorbent assay (ELISA)
- flow cytometry (FACS)
- cell surface radioimmunoassay (RIA)
Biochem/physiol Actions
Multidrug resistance protein 1 (MDR1) functions as an adenosine triphosphate (ATP)-dependent efflux pump. It is also involved in extruding certain xenobiotics and protect cells from toxicants. MDR1 gene is overexpressed in different cancer types including leukemias, lymphomas, myelomas, breast cancer, ovarian cancer, gastrointestinal stromal tumor (GIST), non-small cell lung cancer (NSCLC), fallopian tube, colon, renal, and thyroid cancers. Overexpression of MDR1 gene plays an important role in prompting multidrug resistance in cancer chemotherapy causing high resistance of cancerous cells to a wide variety of substrate anticancer drugs.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Gabriella Spengler et al.
Anticancer research, 36(11), 5701-5706 (2016-10-30)
Thioridazine (TZ), an antipsychotic drug, renders multidrug-resistant (MDR) cancer cells susceptible to cytotoxic agents to which they were initially resistant, has anti-prolilferative activity and apoptosis-inducing properties in various tumor cell lines and cancer stem cells. Whereas the anti-proliferative activity takes
Xing-Duo Dong et al.
Frontiers in pharmacology, 13, 843829-843829 (2022-03-15)
The KRAS-G12C inhibitor ARS-1620, is a novel specific covalent inhibitor of KRAS-G12C, possessing a strong targeting inhibitory effect on KRAS-G12C mutant tumors. Overexpression of ATP-binding cassette super-family B member 1 (ABCB1/P-gp) is one of the pivotal factors contributing to multidrug
Multidrug resistance in breast cancer: a meta-analysis of MDR1/gp170 expression and its possible functional significance
Trock B J, et al.
Journal of the National Cancer Institute, 89(13), 917-931 (1997)
Ziyad Binkhathlan et al.
Current cancer drug targets, 13(3), 326-346 (2013-02-02)
One of the major causes of failure in cancer chemotherapy is multidrug resistance (MDR), where cancer cells simultaneously become resistant to different anticancer drugs. Over-expression of membrane efflux pumps like P-glycoprotein (P-gp) that recognizes different chemotherapeutic agents and transports them
The biochemistry of P-glycoprotein-mediated multidrug resistance.
J A Endicott et al.
Annual review of biochemistry, 58, 137-171 (1989-01-01)
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