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G0762

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-3AF, aqueous solution, ≥60,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
Enzyme Commission number:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Helix pomatia

type

Type H-3AF

form

aqueous solution

specific activity

≥60,000 units/mL

impurities

≤1,000 unit/mL Sulfatase

shipped in

wet ice

storage temp.

2-8°C

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Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Linkage

A further processing of Type H-3 to remove agglutinin.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W Lechner et al.
Acta endocrinologica, 109(2), 266-268 (1985-06-01)
Unconjugated and total oestriol (E3) have been determined in saliva (S) and plasma (P) by radioimmunoassay using a highly specific antiserum against E3. Total oestrogens (E = E1 + E2 + E3) in urine were analysed by the Kober-Ittrich method.
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
Kenneth K C Bramwell et al.
The Journal of clinical investigation, 124(1), 311-320 (2013-12-18)
Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most prevalent arthropod-borne illness in the United States and remains a clinical and social challenge. The spectrum of disease severity among infected patients suggests that host genetics contribute to pathogenic
Tracey B Tucker Zhou et al.
The Journal of biological chemistry, 288(51), 36302-36311 (2013-11-13)
Klotho (KL) is an age-regulating protein named after the Greek goddess who spins the thread of life. Mice deficient in KL are normal throughout development, but rapidly degenerate and display a variety of aging-associated abnormalities that eventually lead to decreased
Regina Goetz et al.
Nature reviews. Molecular cell biology, 14(3), 166-180 (2013-02-14)
Fibroblast growth factors (FGFs) mediate a broad range of functions in both the developing and adult organism. The accumulated wealth of structural information on the FGF signalling pathway has begun to unveil the underlying molecular mechanisms that modulate this system
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Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.

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