G7017
β-Glucuronidase from Helix pomatia
Type HP-2, aqueous solution, ≥100,000 units/mL
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
Helix pomatia
Quality Level
type
Type HP-2
form
aqueous solution
specific activity
≥100,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
shipped in
wet ice
storage temp.
2-8°C
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General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis
Application
- Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
- Removal of glucuronide conjugates such as for the preparation of Baicalein.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Features and Benefits
- pH optima 4.0 - 5.0.
- Sulfatase pH optima 6.2
- Low Sulfatase activity (<7,500 units/mL).
- Ammonium Sulfate suspension.
- Mollusk-derived beta-glucuronidase.
- Useful for acidic hydrolysis of glucuronides in low pH samples.
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Linkage
Similar to G0876, but produced by Sigma.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Protocols
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.
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