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F4680

Sigma-Aldrich

Fluoromount™ Aqueous Mounting Medium

liquid

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

product name

Fluoromount™ Aqueous Mounting Medium, for use with fluorescent dye-stained tissues

Quality Level

200

form

liquid

color

clear

suitability

suitable for immunohistochemistry

application(s)

diagnostic assay manufacturing
hematology
histology

Related Categories

General description

Fluoromount is an aqueous based mounting medium,similar to Gel Mountä, for use with coverslips to preserve tissues stained with fluorescent dyes. Thisproduct is compatible with FITC, phycoerythrin, phycocyanin, and allophycocyanin labeling. It can alsobe used with other fluorescent markers such as TexasRedÒand rhodamine. The medium after use can easily be removed by soaking the slides in deionized water.

Application

Fluoromount™ Aqueous Mounting Medium has been used for mounting:
  • coverslips on slides for immunofluorescence microscopic examination of PC12 and Hela cells
  • hippocampus neurons cells for confocal microscope studies
  • brain sections for immunofluorescence staining

Legal Information

Fluoromount is a trademark of Diagnostic BioSystems, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Cell and Organelle Labeling with Fluorescent Antibodies

The Human Protein Atlas Program has carefully selected three different human cell lines, A-431 epidermoid carcinoma, U-251 MG glioblastoma and U-205 osteosarcoma, for organelle mapping of the proteome. As Prestige Antibodies are studied by immunofluorescence (IF) staining, three well-characterized organelle markers for nuclei, microtubules and endoplasmic reticulum are used correspondingly as specific probes. The high-resolution confocal images are annotated based on sub-cellular localization. In addition, staining intensity and characteristics are also part of an overall assessment as well as comparison to literature (if available).

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