11666681001
Roche
Buffers in a Box, Premixed SSC Buffer, 20x
pH 7.0, pkg of 4 L, solution
Synonym(s):
SSC buffer
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About This Item
UNSPSC Code:
12352200
Recommended Products
form
solution
packaging
pkg of 4 L
manufacturer/tradename
Roche
pH
7.0
shipped in
ambient
storage temp.
20-25°C
General description
20x solution
Application
Buffers in a Box, Premixed SSC Buffer, 20x has been used:
- as a wash buffer in in situ hybridization to determine the expression of miRNAs in paraffin-embedded tissue specimens
- as a wash buffer in single-molecule fluorescent in situ hybridization (smFISH) protocol using U2-OS cells and primary neuronal cultures
- as a component of rolling-circle amplification (RCA) hybridization buffer for use in fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissue samples
Features and Benefits
- Stable 20x solution of saline sodium citrate (SSC) purified by filtration using a 0.2 μm pore size membrane
- resistant to enzymatic and nonenzymatic degradation
- free from nuclease or protease activity
- can store at room temperature
Quality
Absence of contaminants: Free of DNase and RNase according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Malgorzata J Latallo et al.
Methods (San Diego, Calif.), 162-163, 12-22 (2019-03-25)
The central dogma of molecular biology reaches a crescendo at its final step: the translation of an mRNA into its corresponding protein product. This process is highly regulated both spatially and temporally, requiring techniques to interrogate the subcellular translational status
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