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Key Documents

MAB5556

Sigma-Aldrich

Anti-Nicastrin Antibody

ascites fluid, clone 9C3, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9C3, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_015331.2

UniProt accession no.

Q92542

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NCSTN(23385)

Specificity

Nicastrin.

Immunogen

Synthetic peptide corresponding to the C terminal 18 a.a. of mouse Nicastrin. Due to sequence similarity, it is expected that the antibody will react with most mammals.

Application

Anti-Nicastrin Antibody is an antibody against Nicastrin for use in IP, WB & IC.
Western blot. The antibody recognizes a doublet of ~100-110 kDa. Suggested antibody dilution buffer is TBS with 0.1% Tween-20 and 5% non-fat milk powder. Suggested incubation time is overnight at 2-8°C or 1-2 hours at room temperature.

Immunocytochemistry

Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Identification of two novel synaptic ?-secretase associated proteins that affect amyloid ?-peptide levels without altering Notch processing.
Susanne Frykman,Yasuhiro Teranishi,Ji-Yeun Hur,Anna Sandebring,Natsuko Goto Yamamoto et al.
Neurochemistry International null
Cary Esselens et al.
The Journal of cell biology, 166(7), 1041-1054 (2004-09-29)
Presenilin 1 (PS1) interacts with telencephalin (TLN) and the amyloid precursor protein via their transmembrane domain (Annaert, W.G., C. Esselens, V. Baert, C. Boeve, G. Snellings, P. Cupers, K. Craessaerts, and B. De Strooper. 2001. Neuron. 32:579-589). Here, we demonstrate
Jolanta L Lundgren et al.
Journal of neurochemistry, 135(3), 606-615 (2015-08-25)
Synaptic degeneration and accumulation of the neurotoxic amyloid β-peptide (Aβ) in the brain are hallmarks of Alzheimer disease. Aβ is produced by sequential cleavage of the amyloid precursor protein (APP), by the β-secretase β-site APP cleaving enzyme 1 (BACE1) and
gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
Fr?nberg, J; Karlstrom, H; Winblad, B; Tjernberg, LO; Frykman, S
Testing null
Synaptic and endosomal localization of active gamma-secretase in rat brain.
Frykman, S; Hur, JY; Fr?nberg, J; Aoki, M; Winblad, B; Nahalkova, J; Behbahani, H; Tjernberg, LO
Testing null
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