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G005859

HPLC Analysis of Carotenes on Ascentis® Express RP-Amide, Methanol Mobile Phase

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase: methanol
flow rate: 1 mL/min
pressure: 1653 psi (114 bar)
column temp.: 30 °C
detector: UV-VIS, 454 nm
injection: 5 μL
sample: V8 Splash® fruit juice was extracted with hexane, the extract dissolved 1:1 into ethanol for HPLC injection

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Analysis was conducted using juice extract, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
V8 Splash is a registered trademark of CSC Brands, LP

analytical column

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