G004529
HPLC Analysis of Dopa and Carbidopa Enantiomers on Astec® CHIROBIOTIC® T
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] 20 mM ammonium acetate, pH 4; [B] methanol; (30:70, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 5 μL
sample: 1 mg/mL in methanol
suitability
application for HPLC
application(s)
clinical
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Analyte
Description
3,4-Dihydroxy-L-phenylalanine, ≥98% (TLC)
S-(−)-Carbidopa, ≥98% (TLC), powder
3,4-Dihydroxy-D-phenylalanine, powder, ≥95%
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