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80023448

TSKgel® UP-SW3000 HPLC Column

phase diol, L × I.D. 30 cm × 4.6 mm, 2 μm particle size

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About This Item

UNSPSC Code:
41115700

material

stainless steel

Agency

suitable for USP L20
suitable for USP L59

product line

TSKgel®

manufacturer/tradename

Tosoh 0023448

parameter

≤0.5 M salt concentration
0-30% aqueous soluble organic solvents
0.35 mL/min flow rate
10-30 °C temp. range
340 bar pressure

technique(s)

HPLC: suitable

L × I.D.

30 cm × 4.6 mm

matrix

silica particle platform

matrix active group

diol phase

particle size

2 μm

pore size

250 Å

pH range

2.5-7.5

separation technique

size exclusion (SEC)

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General description

TSKgel® UP-SW3000, the new 2 micron UHPLC column with 25 nm pore size, is the latest addition to the renowned TSKgel SW series. TSKgel UP-SW3000 represents the fifth generation of high performance gel filtration columns. They feature the same pore size as the well-established TSKgel G3000SWXL and facilitate method transfer from conventional gel filtration to UHPLC technology. The 30 cm column delivers dramatically increased resolution between fragments, monomers, and aggregates.

Other Notes

Related Catagories: Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Gel Filtration Columns for Proteins and Other Biopolymers, TSKgel Gel Filtration Columns to Analyze Purity and Aggregates, TSKgel Size Exclusion Columns

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Articles

2 Micron SEC Column with Dual Functionality for Use in UHPLC and HPLC Systems

These columns are designed to be operated with a simple and well established method (sodium phosphate mobile phase, pH 6.8) and are packed for use in both HPLC and UHPLC systems.

Analytical Size-exclusion Chromatography Columns for Biomolecule Separations

Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.

Protocols

Analysis of a Bispecific Monoclonal Antibody Using Size Exclusion Chromatography and Mass Spectrometry

Bispecific antibodies are a new class of biotherapeutics where one therapeutic agent can target two different antigens. This application note demonstrates a new size exclusion chromatographic method for characterizing these new biotherapeutics by mass spectrometry.

Workflow for Antibody Aggregate and Fragment Analysis

A complete SEC-UV workflow for quantifying monoclonal antibody fragments, monomers, and aggregates, including procedure for a system suitability test and heat stressing of antibodies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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