X2252

Xanthine Oxidase microbial

lyophilized powder, ≥7 units/mg solid

• Synonym(s): Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase
• CAS Number: 9002-17-9
• Enzyme Commission number: 1.17.3.2 (BRENDA, IUBMB)
• EC Number: 232-657-6
• MDL number: MFCD00082145
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: microbial
• form: lyophilized powder
• specific activity: ≥7 units/mg solid
• mol wt: ~160 kDa
• technique(s): inhibition assay: suitable
• color: brown
• optimum pH: 7.5-8.0
• UniProt accession no.: Q46799; Q46800; Q46801
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)

Description

General description

Research area: Cell Cycle

Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Application

Xanthine Oxidase microbial has been used:

• to determine in vitro xanthine oxidase activity
• as a source to generate superoxide anions during superoxide dismutase assay
• as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction

Biochem/physiol Actions

Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.

Also contains proteolytic activity.

Physical properties

Inhibitors: Reducing agents, Hg⁺⁺, Ag⁺,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65^oC
pH Stability: pH 6.5 - 9.0 (25^oC, 15hr)
Thermal stability: below 55^oC (pH 8.0, 30min)

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Lyophilized powder containing BSA and sodium glutamate as stabilizers

Storage and Stability

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves