T6763
Trypsin Acetylated from bovine pancreas
Type V-S, ≥8,500 BAEE units/mg protein (biuret)
Synonym(s):
Acetyltrypsin
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
biological source
bovine pancreas
Quality Level
type
Type V-S
form
powder
specific activity
≥8,500 BAEE units/mg protein (biuret)
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... TRYP8(282603)
Related Categories
General description
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
Biochem/physiol Actions
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
Unit Definition
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
Preparation Note
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
Other Notes
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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