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T5251

Sigma-Aldrich

D-(+)-Trehalose dihydrate

≥98.5% (HPLC), from Saccharomyces cerevisiae

Synonym(s):

α,α-Trehalose dihydrate, D-Trehalose dihydrate, α,α-Trehalose, α-D-Glucopyranosyl-α-D-glucopyranoside

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About This Item

Empirical Formula (Hill Notation):
C12H22O11 · 2H2O
CAS Number:
Molecular Weight:
378.33
Beilstein:
5322018
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.25

biological source

Saccharomyces cerevisiae

Assay

≥98.5% (HPLC)

form

powder

optical activity

[α]/D 174 to 182 °, c = 1.0% (w/v) in water

technique(s)

HPLC: suitable
cryopreservation: suitable

mp

97-99  °C

application(s)

agriculture
agriculture
cell analysis
genomic analysis
life science and biopharma

SMILES string

[H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1

InChI key

DPVHGFAJLZWDOC-PVXXTIHASA-N

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General description

D-(+)-Trehalose, a disaccharide, acts as a chemical chaperone and serves as a carbohydrate reserve in microorganisms. It functions as an osmolyte, safeguarding cells against various environmental stresses. Further, it also plays a crucial role in protein stabilization during freeze drying, making it a valuable asset in the areas of biochemical, cell culture, and excipient research

Application

  • Use as a cryoprotectant in a variety of cell freezing media
  • Used in excipeint research

Biochem/physiol Actions

Trehalose is a non-reducing sugar formed from two glucose units joined by a 1-1 alpha bond. It is thought to provide plants and animals with the ability to withstanding periods of dehydration.

Features and Benefits

  • Prepared from Saccharomyces cerevisiae
  • Suitable for use with HPLC and GC-MS(4)

Preparation Note

Prepared by the modified procedure of Payen, R., Can. J. Res., 27B, 749 (1949).

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sandra Jesus et al.
AAPS PharmSciTech, 19(1), 101-113 (2017-06-15)
This report extensively explores the benefits of including chitosan into poly-ε-caprolactone (PCL) nanoparticles (NPs) to obtain an improved protein/antigen delivery system. Blend NPs (PCL/chitosan NPs) showed improved protein adsorption efficacy (84%) in low shear stress and aqueous environment, suggesting that
Alan Twomey et al.
International journal of pharmaceutics, 487(1-2), 91-100 (2015-04-19)
In frozen and lyophilized systems, the biological to be stabilized (e.g. therapeutic protein, biomarker, drug-delivery vesicle) and the cryo-/lyo-protectant should be co-localized for successful stabilization. During freezing and drying, many factors cause physical separation of the biological from the cryo-/lyo-protectant
Sabine Ullrich et al.
Journal of pharmaceutical sciences, 104(6), 2040-2046 (2015-04-03)
The importance of cake adhesion to the inside vial wall during lyophilization of amorphous trehalose cakes was determined by using hydrophobized vials. The degrees of cake shrinkage and cracking were determined independently by photographic imaging of the cake top surface
Joachim Schaefer et al.
International journal of pharmaceutics, 489(1-2), 124-130 (2015-05-06)
The inactivation of catalase during spray-drying over a range of outlet gas temperatures could be closely represented by the Arrhenius equation. From this an activation energy for damage to the catalase could be calculated. The close fit to Arrhenius suggests
Anke Sass et al.
Drug development and industrial pharmacy, 40(6), 749-757 (2013-04-20)
The spray-drying behaviour of 16 water-miscible organic solvents on a bench-scale machine (Büchi B290 with inert loop) was determined under mild-to-moderate process conditions, namely inlet gas temperature of 130 °C and liquid feed flow rate of ≤3 mL/min. The solvents with boiling

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