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T4444

Sigma-Aldrich

3, 3′,5 ,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, aqueous solution

Synonym(s):

TMB

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About This Item

CAS Number:
54827-17-7
MDL number:
MFCD00007748
UNSPSC Code:
12352204
PubChem Substance ID:
24900209
NACRES:
NA.83

product name

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution

Quality Level

200

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

Related Categories

General description

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Application

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:
  • to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant
  • to measure the levels of α-hemolysin toxin
  • in imaging bound IgGs

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The activity of hyaluronan synthase 2 is regulated by dimerization and ubiquitination
Karousou E, et al.
The Journal of Biological Chemistry, 285(31), 23647-23654 (2010)
Ruud van den Bos et al.
Behavioural pharmacology, 30(2 and 3-Spec Issue), 260-271 (2019-02-07)
Maternal stress and early life stress affect development. Zebrafish (Danio rerio) are ideally suited to study this, as embryos develop externally into free-feeding larvae. The objective of this study was therefore to assess the effects of increased levels of cortisol
Kate M Smith et al.
Biochimica et biophysica acta. Molecular cell research, 1865(8), 1114-1129 (2018-05-12)
Nuclear import involves the recognition by importin (IMP) superfamily members of nuclear localization signals (NLSs) within protein cargoes destined for the nucleus, the best understood being recognition of classical NLSs (cNLSs) by the IMPα/β1 heterodimer. Although the cNLS consensus [K-(K/R)-X-(K/R)
E Brandon Strong et al.
Scientific reports, 9(1), 7-7 (2019-01-11)
Microfluidic paper-based analytical devices (microPADs) are emerging as cost-effective and portable platforms for point-of-care assays. A fundamental limitation of microPAD fabrication is the imprecise nature of most methods for patterning paper. The present work demonstrates that paper patterned via wax
Granulocyte/macrophage-colony-stimulating factor autoantibodies and myeloid cell immune functions in healthy subjects
Uchida K, et al.
Blood, 113(11), 2547-2556 (2009)
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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