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T0678

Sigma-Aldrich

Anti-Tryptophan Hydroxylase Antibody

mouse monoclonal, WH-3

Synonym(s):

Anti-TPH

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About This Item

MDL number:
MFCD00282500
UNSPSC Code:
12352203
NACRES:
NA.41

product name

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, clone WH-3, ascites fluid

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

WH-3, monoclonal

mol wt

antigen 55 kDa

contains

15 mM sodium azide

species reactivity

human, monkey, rabbit, rat

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract

isotype

IgG3

UniProt accession no.

P17752

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TPH1(7166) , TPH2(121278)
rat ... Tph1(24848) , Tph2(317675)

Related Categories

General description

Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.
The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.

Specificity

This antibody reacts specifically with tryptophan hydroxylase.

Immunogen

recombinant rabbit tryptophan hydroxylase.

Application

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunoblot
  • immunocytochemistry

Biochem/physiol Actions

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pricing

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Distinct neurochemical and functional properties of GAD67-containing 5-HT neurons in the rat dorsal raphe nucleus
Shikanai H, et al.
The Journal of Neuroscience, 32(41), 14415-14426 (2012)
Neuronal loss in the rostral ventromedial medulla in a rat model of neuropathic pain
Leong ML, et al.
The Journal of Neuroscience, 31(47), 17028-17039 (2011)
Guillaume P Dugué et al.
PloS one, 9(8), e105941-e105941 (2014-08-26)
The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro
Chao-Chuan Wang et al.
The international journal of neuropsychopharmacology, 16(9), 2027-2039 (2013-07-05)
Accumulating evidence suggests that dysfunction of the amygdala is related to abnormal fear processing, anxiety, and social behaviors noted in autistic spectrum disorders (ASDs). In addition, studies have shown that disrupted brain serotonin homeostasis is linked to ASD. With a
Anthony C Ruocco et al.
Neuropsychology, 30(1), 18-27 (2015-12-29)
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the formation of 5-hydroxytryptophan, a precursor to the neurotransmitter serotonin. Variations in the gene encoding this enzyme may underlie difficulties in impulse control; however, the proximate relationship between risk alleles for polymorphisms in
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