SRP6105

MDH from bacteria

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): ECK3225, JW3205, Malate dehydrogenase
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: bacterial
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 34.9 kDa (336 aa, 1-312 aa + NT His Tag)
• packaging: pkg of 100 μg
• technique(s): activity assay: suitable
• UniProt accession no.: P61889
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: Escherichia coli ... MDH(947854)

Description

General description

Malate dehydrogenase (MDH) belongs to the LDH/MDH superfamily and MDH type 1 family. It exists as a homodimer and tetramer in prokaryotes.

Biochem/physiol Actions

Malate dehydrogenase (MDH) catalyzes the conversion of malate into oxaloacetate (using NAD⁺) and vice versa (reversible reaction). This reaction is part of many metabolic pathways, including the citric acid cycle. MDH has a key role in the central oxidative pathway. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules.

Physical form

1 mg/mL solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, 50 mM NaCl and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable