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SAE0045

Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Synonym(s):

Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

human (human Rhinovirus Type 14)

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

aqueous solution

specific activity

≥5000 U/mg

mol wt

21 kDa

concentration

0.8-1.2 mg/mL

technique(s)

protein purification: suitable

suitability

suitable for protein modification

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−20°C

General description

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Application

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Unit Definition

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Physical form

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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