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SAB4700594

Sigma-Aldrich

Monoclonal Anti-CD90.2, azide free antibody produced in mouse

clone 1aG4/C5, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Thy-1.2, Anti-Thy1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1aG4/C5, monoclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG3

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Thy1(21838)

General description

Cluster of differentiation 90.2 (CD90.2), also known as Thy-1.2, is a 25-37kDa GPI-anchored protein, encoded by the gene mapped to mouse chromosome 9. The protein is mainly localized on the external leaflet of the lipid bilayer and lipid rafts. The encoded protein is one of the allele of CD90 and is expressed primarily on murine T-cells.
The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI linked glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.

Immunogen

thymocytes from C3H/Di mice (Thy-12 positive)

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Cluster of differentiation 90.2 (CD90.2) plays a major role in T-cell activation. In addition, it is also implicated in various nonimmunologic functions such as, inhibition of neurite outgrowth, apoptotic signaling, leukocyte and melanoma cell adhesion and migration, tumor suppression and fibroblast proliferation and migration. CD90. also acts as a tumor suppressor in ovarian cancer and nasopharyngeal carcinoma (NPC). Deficiency of CD90 is associated with the development of pulmonary fibrosis. The protein expressed in choroidal endothelial cells (CECs) modulates VEGF-mediated CEC activation and migration.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in azide free HEPES buffer, pH 7

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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John E Bradley et al.
BioFactors (Oxford, England), 35(3), 258-265 (2009-05-08)
Thy-1 or CD90 is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype; however
Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis.
Rege TA and Hagood JS
Faseb Journal, 20, 1045-1054 (2006)
TGF-β1 epigenetically modifies Thy-1 expression in primary lung fibroblasts
Neveu WA
American Journal of Physiology. Cell Physiology, 309, C616-C626 (2015)
Thy-1 Regulates VEGF-Mediated Choroidal Endothelial Cell Activation and Migration: Implications in Neovascular Age-Related Macular Degeneration
Wang H
Investigative Ophthalmology & Visual Science, 57, 5525-5534 (2016)

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