SAB4600465

Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, CF^™405M antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 100
• conjugate: CF^™ 405M conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: chicken
• concentration: ~2 mg/mL
• technique(s): flow cytometry: 1-10 μg/mL; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1-10 μg/mL; western blot: suitable
• fluorescence: λ_ex 408 nm; λ_em 452 nm
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin Y is an antibody produced in birds. IgY is similar to mammalian IgG but differs in some characteristics from IgG. IgY does not bind with mammalian complements nor rheumatoid factor, which are present in mammalian serum and anti-mammalian IgG antibodies. During oogenesis in chicken, IgY can pass across the follicular epithelium of the ovary and aggregate in the yolk. The heavy chain of IgY and mammalian IgG has a molecular weight of 68kDa and 50kDa, respectively. The structure of mammalian IgG is considered more stable than that of IgY. IgG antibodies, which often cross-react to IgG from different mammalian species, exhibit low cross-reactivity to IgY. Thus, IgY is beneficial when it is used as a biochemical/clinical reagent.

This product is prepared by labeling highly cross-adsorbed goat anti-chicken IgY (IgG) (H+L) with CF405M. CF405M is spectrally similar to and as bright as Pacific Blue dye but is significantly more photostable than the latter. An additional advantage of CF405M is that it has less spillover in the 525/50 green channel than Pacific Blue dye due to narrower emission peak, making CF405M a superior choice in multi-color detection applications. To minimize cross-reactivity, the antibody has been adsorbed against Bovine, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep serum. 

Specificity

Binds all chicken IgYs (minimal cross-reaction with bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep proteins)

Immunogen

chicken IgY (H+L)

Application

Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, CF^™405M antibody produced in goat has been used in immunohistochemistry.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable