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Key Documents

SAB4504467

Sigma-Aldrich

Anti-phospho-MEK1/2 (pSer221) antibody produced in rabbit

affinity isolated antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 43 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:20000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P36507
Q02750

UniProt accession no.

P36507

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer221)

Gene Information

human ... MAP2K1(5604) , MAP2K2(5605)

Immunogen

The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser221.

Immunogen Range: 193-242

Biochem/physiol Actions

MAP2k1/2 (dual specificity mitogen-activated protein kinase kinase 1/2) is a serine threonine kinase and is required for ERK (extracellular-signal-regulated kinase) activation. Activation of ERK is associated with production of IL-10 (interleukin 10) and IL-12. Absence of the Map2k1 gene results in embryonic lethality. Map2k1 is responsible for the stimulation of epidermal proliferation, regulating cell migration in fibroblasts. Deficiency of Map2k1 protein causes lupus-like syndrome. The gene encoding Map2k1 is localized on human chromosome 15q22.31 and the MAP2K2 gene is localized on human chromosome 19p13.3.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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MEK1 dependent and independent ERK activation regulates IL-10 and IL-12 production in bone marrow derived macrophages.
Bouhamdan M, et al.
Cellular Signalling, 27(10), 2068-2076 (2015)
The RASopathies: developmental syndromes of Ras/MAPK pathway dysregulation
Tidyman WE and Rauen KA
Current Opinion in Genetics & Development, 19(3), 230-236 (2009)

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