SAB4200571

Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal

clone NSE-P1, purified from hybridoma cell culture

• Synonym(s): Anti-2-phospho-D-glycerate hydrolyase, Anti-Enolase 2 (gamma, neuronal), Anti-NSE, Anti-Neural enolase, Anti-gamma-enolase, Monoclonal Anti-2-phospho-D-glycerate hydro-lyase
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NSE-P1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~47 kDa
• species reactivity: human, rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.; western blot: 0.5-1.0 μg/mL using NTERA-2 (NT2/D1) total cell extracts.
• isotype: IgG1
• UniProt accession no.: P09104
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ENO2(2026)

Description

General description

Monoclonal anti-neuron-specific enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Neuron specific enolase (NSE) belongs to the family of enolase enzymes. Enolases have three subunits (a, b, and g), which can combine to form five different isoenzymes: aa, ab, ag, bb, and gg. Enolase 1 (aa) is present in adipose tissue, kidney, liver, and spleen. Enolase 3 (bb) is muscle specific. Enolase 2 (ENO2), also known as neuron-specific enolase (NSE) is a cytoplasmic enzyme, that is released during cell destruction and has a half-life of approximately 30 hours in serum. This dimeric enzyme (gg) is found in neurons and in neuroendocrine cells. ENO2 gene is mapped to human chromosome 12p13.

Specificity

Monoclonal Anti- Neuron-Specific Enolase (NSE) recognizes human, rat, and mouse NSE.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminal region of human NSE.¹ The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal has been used in:

• immunofluorescence staining
• immunocytochemistry
• immunofluorescence microscopy
• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

Biochem/physiol Actions

Enolase 2 (ENO2) participates in glycolysis by converting β-glycerophosphate into dihydroxyacetone phosphate. It serves as a neurobiochemical marker of brain damage after traumatic brain injury, anoxic encephalopathy, stroke, and cardiac arrest. NSE also acts as a circulating marker for neuroendocrine tumors. High levels of NSE is observed in breast cancer upon exposure to arsenite and cadmium.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable