P6649
Protein A–Sepharose™ 6MB
aqueous ethanol suspension
Synonym(s):
Protein A–Agarose macrobeads
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About This Item
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form
aqueous ethanol suspension
extent of labeling
~1 mg per mL
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~6 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
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General description
P6649-10ml′s updated product number is GE17-0469-01
Application
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A–Sepharose™ 6MB has been used in immunoprecipitation.
Physical form
Suspension in 20% ethanol
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
100.4 °F
Flash Point(C)
38 °C
Certificates of Analysis (COA)
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G V Hillyer et al.
The American journal of tropical medicine and hygiene, 38(3), 547-552 (1988-05-01)
Total RNA containing messenger RNA has been isolated from adult Fasciola hepatica and translated in vitro using the rabbit reticulocyte lysate system. L-[35S]-methionine labeled translation products have been immunoprecipitated with sera collected from rabbits infected with F. hepatica, rabbits immunized
Sundar Rajan Selvaraj et al.
Molecular biology of the cell, 19(12), 5579-5592 (2008-09-26)
Retinol-binding protein (RBP) is secreted out of the cell in its ligand-bound holo-form. The apo-form of RBP is selectively retained within the endoplasmic reticulum (ER) by a mechanism that remains unknown. Using isolated microsomal system, we have recapitulated the biogenesis
L F Wang et al.
Gene, 169(1), 53-58 (1996-02-22)
Epitope tagging (Eta) is becoming an increasingly useful technique in molecular biology and biotechnology for the detection, characterisation and purification of recombinant proteins (re-proteins). Here we describe a novel Eta system composed of two different monoclonal antibodies (mAb; D11 and
Gaurav Dugar et al.
Molecular cell, 69(5), 893-905 (2018-03-03)
Cas9 nucleases naturally utilize CRISPR RNAs (crRNAs) to silence foreign double-stranded DNA. While recent work has shown that some Cas9 nucleases can also target RNA, RNA recognition has required nuclease modifications or accessory factors. Here, we show that the Campylobacter
A J Williams et al.
Diabetes care, 24(3), 504-509 (2001-04-06)
To determine the extent of celiac autoimmunity in type 1 diabetic patients and the overlap between islet and celiac autoimmunity in their nondiabetic relatives. IgA antibodies to tissue transglutaminase were determined in serum taken from 433 type 1 diabetic patients
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