N5661
Nuclease S1 from Aspergillus oryzae
for single-strand DNA/RNA digestion
Synonym(s):
Endonuclease S1
About This Item
Recommended Products
biological source
Aspergillus sp. (A. oryzae)
Quality Level
form
solution
concentration
≥100000 units/mL
technique(s)
DNA purification: suitable
suitability
suitable for nucleic acid purification
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
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General description
Application
Biochem/physiol Actions
Unit Definition
Physical form
Kit Components Only
- 30mM Sodium acetate .25-.25 %
- 50mM Sodium chloride .29 %
- 1mM Zinc chloride .01 %
- Glycerol 50 %
- 2mg/mL Protein .2 %
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
To measure nuclease S1 activity, a spectrophotometric rate-determination assay is used at 260 nm. One unit of enzyme will cause 1 μg of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 and 37 °C.
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