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MWGF200

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da

Synonym(s):

Gel Filtration Protein Ladder

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

form

solid

Quality Level

200

technique(s)

liquid chromatography (LC): suitable

storage temp.

−20°C

General description

Gel filtration markers kit contains five vials of proteins and 1 vial of blue dextran for calibration.

Application

Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit Components Also Available Separately

Product No.
Description
SDS
  • A8531Albumin, bovine serum, 66,000 Da MW 1 mL/vialSDS
  • A8656Alcohol Dehydrogenase, yeast, 150,000 Da MW 1 mL/vialSDS
  • A8781β-Amylase, sweet potato, 200,000 Da MW 1 mL/vialSDS
  • D4772 Blue dextran, 2,000,000 Da MW 1 mL/vialSDS
  • C7025Carbonic Anhydrase, bovine erythrocytes 29,000 Da MW 1 mL/vialSDS
  • C7150Cytochrome c, horse heart, 12,400 Da MW 1 mL/vialSDS

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ulrich Strych et al.
BMC microbiology, 7, 40-40 (2007-05-19)
Over the past fifteen years, antibiotic resistance in the Gram-positive opportunistic human pathogen Streptococcus pneumoniae has significantly increased. Clinical isolates from patients with community-acquired pneumonia or otitis media often display resistance to two or more antibiotics. Given the need for
Cristina L Marolda et al.
Journal of bacteriology, 190(6), 2128-2137 (2008-01-22)
Wzz is a membrane protein that determines the chain length distribution of the O-antigen lipopolysaccharide by an unknown mechanism. Wzz proteins consist of two transmembrane helices separated by a large periplasmic loop. The periplasmic loop of Escherichia coli K-12 Wzz
Andreas Krug et al.
The Journal of biological chemistry, 280(1), 585-595 (2004-10-21)
In Corynebacterium glutamicum, the activity of aconitase is 2.5-4-fold higher on propionate, citrate, or acetate than on glucose. Here we show that this variation is caused by transcriptional regulation. In search for putative regulators, a gene (acnR) encoding a TetR-type
Fiona McArthur et al.
Journal of bacteriology, 187(15), 5292-5300 (2005-07-21)
The core oligosaccharide component of the lipopolysaccharide can be subdivided into inner and outer core regions. In Escherichia coli, the inner core consists of two 3-deoxy-d-manno-octulosonic acid and three glycero-manno-heptose residues. The HldE protein participates in the biosynthesis of ADP-glycero-manno-heptose
Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.
Maharjan S, Singh B, Bok JD, et al.
PLoS ONE, 9(5), e96259-e96259 (2014)
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