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MSP14

Sigma-Aldrich

Membrane Scaffold Protein 1D1 DW

recombinant, expressed in E. coli, Dark MSP

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

recombinant

expressed in E. coli

Assay

≥90% (SDS-GE)

form

buffered aqueous solution

shipped in

ambient

storage temp.

−20°C

General description

Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).

The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The MSP1ΔW variant of MSP1D1, sometimes referred to as “Dark MSP”, replaces the two tryptophan (W) residues in the parent MSP1D1 sequence with phenylalanines (F). These two (W→F) substitutions are at the following positions of the original native A-1 sequence:
  • Position 72, within the Helix 2 (H2) segment
  • Position 108, within the Helix 4 (H4) segment

Application

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Legal Information

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
  • 7,691,414 Membrane scaffold proteins
  • 7,662,410 Membrane scaffold proteins and embedded membrane proteins
  • 7,622,437 Tissue factor compositions and methods
  • 7,592,008 Membrane scaffold proteins
  • 7,575,763 Membrane scaffold proteins and tethered membrane proteins
  • 7,083,958 Membrane scaffold proteins
  • 7,048,949 Membrane scaffold proteins

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

M7074

Membrane Scaffold Protein 1E3D1

Protocols

Protocols for Membrane Scaffold Proteins and Nanodisc Formation

Nanodisc technology offers a means to overcome some of the challenges associated with solubilizing membrane proteins. Nanodisc technology is applicable to a wide variety of membrane proteins and protein classes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service