MAK332

Pyruvate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Pyruvic Acid Detection Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• input: biological sample(s)
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): gastrointestinal diseases; cancer
• storage temp.: −20°C

Description

General description

Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.

Application

The Pyruvate Assay Kit may be used for:

• Drug Discovery
• Food and Beverage Testing
• Neurological Disease Research

Features and Benefits

Broad Detection Range: Detect Pyruvate levels accurately across a wide range, from 2 µM to 500 µM, using only 10 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.



Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.



Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for pyruvate detection in biological samples and for studying the effects of drugs on pyruvate metabolism.

Principle

The Pyruvate Assay Kit uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorometric assays.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C