MAK176
Granzyme B Activity Assay Kit
Sufficient for 100 Fluorometric tests
Synonym(s):
CTLA1, GZMB Activity Assay Kit
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About This Item
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usage
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
hematological disorder; immunological diseases; cancer
storage temp.
−20°C
Gene Information
human ... GZMB(3002)
mouse ... GZMB(14939)
rat ... GZMB(171528)
General description
GZMB codes for granzyme B.
Granzyme B (gzm B) is a peptidase that induces cytotoxic T lymphocyte (CTL)-mediated DNA fragmentation and apoptosis in allogeneic target cells. It cleaves after aspartic acid residues in substrates. Granzyme B-mediated release of mitochondrial cell death factors are required for the activation of caspase-3. Furthermore, the degradation of granzyme B is known to decrease the susceptibility of cancer cells to NK-cell-mediated lysis during hypoxia. Granzyme B activity assays can be used for studying T cell immunity, CTL responses, cell-mediated cytotoxicity, and apoptosis.
Gzm B is a pro-apoptotic member of the granzyme family. It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells.
Gzm B is a pro-apoptotic member of the granzyme family. It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells.
Application
- A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling: Describes a technological advancement where the Granzyme B Activity Assay Kit is integrated into a microfluidic platform, enabling high-throughput and precise measurements of Granzyme B activity at the single-cell level, applicable in both research and clinical diagnostics (Briones et al., 2020).
Suitability
Suitable for the detection of granzyme B activity in biological samples and for the characterization of granzyme B inhibitors.
Principle
Granzyme B activity is determined using an enzyme-catalyzed reaction in which the non-fluorescent substrate, Ac-IEPD-AFC, is hydrolyzed resulting in the release of AFC (λex = 380/λem = 500 nm) proportional to the enzymatic activity present. One unit of granzyme B is the amount of enzyme that hydrolyzes 1 pmole of the substrate per minute at 37 °C.
Kit Components Only
Product No.
Description
- Granzyme B Assay Buffer
- Granzyme B Substrate
- Granzyme B Positive Control
- AFC Standard, 1 mM
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
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Granzyme B?mediated cytochrome c release is regulated by the Bcl-2 family members bid and Bax.
Heibein J A, et al.
The Journal of Experimental Medicine, 192(10), 1391-1402 (2000)
Granzyme B enters the mitochondria in a Sam50-, Tim22-and mtHsp70-dependent manner to induce apoptosis.
Chiusolo V, et al.
Cell Death and Differentiation, 24(4), 747?758-747?758 (2017)
Granzymes: a family of lymphocyte granule serine proteases.
Trapani J A.
Genome Biology, 2(12), 3014-3011 (2001)
Granzyme B degradation by autophagy decreases tumor cell susceptibility to natural killer-mediated lysis under hypoxia.
Baginska J, et al.
Proceedings of the National Academy of Sciences of the USA, 201304790-201304790 (2013)
Cytotoxic lymphocytes require granzyme B for the rapid induction of DNA fragmentation and apoptosis in allogeneic target cells.
Heusel J W, et al.
Cell, 76(6), 977-987 (1994)
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