M1255

Calcein Blue

fluorescent dye

• Synonym(s): 4-Methylumbelliferone-8-methyliminodiacetic acid
• Empirical Formula (Hill Notation): C₁₅H₁₅NO₇
• CAS Number: 54375-47-2
• Molecular Weight: 321.28
• EC Number: 259-129-8
• MDL number: MFCD00006867
• UNSPSC Code: 12171500
• PubChem Substance ID: 24896629
• NACRES: NA.47

Properties

• Quality Level: 200
• form: powder
• solubility: 1 M NaOH: 50 mg/mL
• fluorescence: λ_ex 322 nm; λ_em 445 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
• InChI: 1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
• InChI key: RJPSHDMGSVVHFA-UHFFFAOYSA-N

Description

General description

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Application

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves