H7290

Anti-HtrA2 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-High Temperature Requirement protein A2, Anti-HtrA serine peptidase 2, Anti-Omi, Anti-PARK13, Anti-PRSS25, Anti-Protease, Serine, 25
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~36 kDa (also 50 kDa band corresponding to HtrA2 precursor)
• species reactivity: human, rat
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): western blot: 2-4 μg/mL using rat liver microsomal fraction and HEK-293 cells expressing human HTRA2
• UniProt accession no.: O43464
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HTRA2(27429) ; rat ... Htra2(297376)

Description

General description

High-temperature requirement protein A2 (HtrA2) belongs to the HtrA family of serine proteases. This protein is located in the mitochondria and is made up of N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM), a single C-terminal PDZ domain that mediates protein-protein interaction, and a conserved catalytic domain of serine proteases. The HTRA2 gene is located on the human chromosome at 2p13.1.

Specificity

Anti-HtrA2 specifically recognizes human and rat HtrA2.

Application

Anti-HtrA2 antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

High-temperature requirement A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. This protein possesses protease activity, which is required for mitochondrial homeostasis. HtrA2 is released from the mitochondria as a mature form in response to apoptotic stimuli and is involved in regulating apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2 promotes cytochrome c-dependent caspase activation by neutralizing inhibitors of apoptosis proteins (IAPs). Mutations that inactivate HtrA2 are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves